Nephrotoxicity
Often kidney-specific injury assessment may not always give the desired results. It may be due to finding the lead optimization of the drug candidate, and undesired safety issues that lead to expensive failures at the beginning of the drug discovery process can be disheartening. However, at Stellixir Biotech, we have strategized nephrotoxicity services provided by an expert team. We look into finding the best in vitro cell-based screening models that will enable your study to assess the nephrotoxicity of compounds tested on the tissue of cells derived from human kidneys.
Our job is to work on the safety evaluation; hence we follow two image-based high content analyses, also known as HCA assays. These are done using human renal proximal tubule epithelial cells (HRPTepiC). We enable to get detailed mechanistic information possible through multiple endpoint analyses, which are overly sensitive. After a thorough assessment by our experts, we give you the underlying toxicity report.
Vital Information Regarding Nephrotoxicity Assessment
- High Content Analysis, cytotoxicity parameters:
- Cell viability: decrease in DAPI (nuclear dye) stained cells
- Apoptosis: increased staining with an anti-active caspase antibody
- Mitotic block - increased staining with an anti-phospho-histone H3 antibody
- 72 hr compound incubation
Vital Information Regarding Cellular Stress Assessment
- High Content Analysis, cellular stress parameters:
- Cell viability: decrease in DAPI (nuclear dye) stained cells
- Cellular stress: increased staining using an anti-HSP27 antibody
- Kidney injury: increased staining using an anti-KIM-1 antibody
- 48 hr compound incubation