Oncology
The drug discovery and development process is a long journey that can be quite costly with high rates of attrition. Many drug candidates will fail during clinical trials, leading to the loss of millions of dollars and multiple years of work. Stellixir, with its expertise in cell culture models, plays an important role for companies in the early stages of drug discovery and even into the preclinical stage. Our In vitro cell culture models and assays offer a wide range of advantages that are beneficial to drug discovery. These assays cost less than running animal studies and can also save time and money by requiring fewer animal studies before going into human clinical trials. There are two categories for these cell culture assays, 2D vs 3D models. Each model has its own advantages and disadvantages depending on what type of evaluation or analysis needs to be performed. We help you from the basic research to every stage as Stellixir Biotech uses an integrated approach for successful research and development in oncology.
Available Discovery Assays:
Cytotoxicity & Proliferation Assays
- SRB assay
- ATP assay
- LDH assay
- Cell cycle
- MTT assay
- Ki67 assay,
- Clonogenic assay
Apoptosis and DNA Damage Studies
- Annexin V/PI assay
- Ao/EtBr assay
- p53 assay
- Caspases assay
- 21 assay
- Comet assay
- MMP - JC1 assay
- Cyt-C assay
- Cell cycle arrest
- NO analysis
- TUNEL assay
- ROS analysis
Angiogenesis and Metastasis Assays
- Cell migration assays
- Scratch assay
- Tube formation assay
- Angiogenesis signalling pathways (HIF1α, TGF-b, VEGF, SMAD, p38, Pi3)
Cancer Signalling Pathway Marker Studies
- C-Jun test
- cAMP assay
- HIF-1 alpha assay
- STAT 1,2,3, & 6 assay
- Phospho-mTOR assay
- Phosphor-Akt (p-Akt) assay
- P53 expression
- Phosphor-ERK assay
- Ca+2 assay
- NF-kappa B/p65
- Phosphor-CREB assay
- EGFR assay
- Akt assay
- P38 activity assay
Available Models:
- Monolayer and 3D cultures using tumor cell lines and primary tumor cells
- Co-Culture with endothelial cells
- Tri-Culture with stromal and endothelial cells
- Tri-Culture with stromal, endothelial, and immune cells
- Hypoxic models
- 3D Organoids of single and co-cultured cells
Techniques Used: Flow Cytometry, Confocal/Fluorescence Microscopy (Multi-colour Immunofluorescence), ELISA (Fluorimetry/Colorimetry), RTqPCR, WB.