Training & Workshop

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Flow Cytometry Workshop

Flow Cytometry Workshop
The workshop will cover the following aspects -
  • Basics of flow cytometry and its applications
  • Sample preparation
  • Analysis and interpretation of acquired data

The modules offered are discussed in detail below, and any one of the standard flow cytometry assays shall be demonstrated.

Hematopoietic and Mesenchymal Stem Cells

Enumeration of CD34+ hematopoietic stem and progenitor cells in bone marrow, cord blood and mobilized peripheral blood. We will discuss techniques on identifying mesenchymal stromal cells and tumor stem cells.

DNA Content and Cell Cycle Analysis

Measurement of cellular DNA content, cell cycle distribution, CDKs analysis, and effect of drugs on cell cycle will be discussed and demonstrated.

Apoptosis and Necrosis Detection

We will discuss the differences between apoptosis and necrosis, and demonstrate assays for Annexin V, caspase activation, apoptosis protein markers viz., Bax, Bcl-2, p53.

Stem Cell Assays and Phenotype Analysis

We will demonstrate and discuss sample processing and staining protocols used for phenotype analysis of hematopoietic malignancies and cell surface marker analysis of tumor stem cell antigens

Functional Assays

Detection of ROS formation, hypoxia signaling, mitochondrial membrane potential, intracellular glutathione content, mitochondrial superoxide generation and viability

Address

No.V-31, 2nd floor, 10th Main Road, Peenya 2nd Stage Industrial Area, Bangalore - 560058, India

Contacts

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+91 - 89718 67345
Email : info@stellixir.com

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